recombinant strain造句

例句与造句

1. The pichia pastoris recombinant strain was induced by methanol addition to the culture medium
   Sds - page分析表明，在63kd处有明显的蛋白质特征带。
2. Expression of cyt1aa from bacillus thuringiensis in bacillus thuringiensis var . tenebrionis strain and the insecticidal activity of the recombinant strain
   基因在拟步甲亚种菌株中的表达及重组菌株杀虫活性研究
3. The gene recombinant strain no . 42 ca n ' t generate ampramycin , which indicated that the cloned gene is involved in apramycin biosynthesis in s . tenebrarius
   通过接合转移的方法将质粒pzxb014导入黑暗链霉菌h6中，筛选基因发生重组的菌株。
4. Finally , the growth conditions , growth curves , fermentation conditions of recombinant strains and the biological properties of purified recombinant protein were investigated
   最后对重组菌的生长条件、生长曲线、发酵条件和表达产物的体外生物学活性进行了研究。
5. Some important factors of high density fermentation of genetic engineering microorganisms including the construct of recombinant strains , culture conditions , growth inhibitor and the process control were described
   摘要阐述了基因工程菌高密度发酵工艺的几个主要影响因素，包括重组菌构建、培养条件、生长抑制因数以及它们的控制技术。
6. It's difficult to find recombinant strain in a sentence. 用recombinant strain造句挺难的
7. Object : the culture medium and culture conditions of psb will be optimized and gene ubia will be cloned for the construction of recombinant strain producing and foundation of the fermentative technology in large scale
   目的：优化psb产coq _ ( 10 )培养基及培养条件和克隆ubia基因，为获取高产coq _ ( 10 )基因工程菌及确定规模化发酵工艺奠定良好的基础。
8. The three recombinant strains were induced to express with iptg . the tannase activity was detected by ultravillet spectroscopy and the target protein was found in sds - page with the correct size of 63kd . the gene was also insered in the plasmid ppic9k of pichia pastoris
   将单宁酶基因tan重组到trc启动子控制下的表达载体pse380中，并分别转化入大肠杆菌dh5 ， top10 ， bl21 ( de3 )三种重组表达菌株中。
9. One highly productive and genetically stable recombinant strain named e - 22 , which produced phytase with 143958 . 3u / ml under the condition of flask cultivation , was selected through further screening . the phytase activity of e - 22 was 341 . 13 times as high as that of the original strain ( 422u / ml )
   经摇瓶复筛后得到l株产酶活性为143958 . 3uzml发酵液，并且具有良好遗传稳定性的高产工程菌株( e22 ) ，其产酶活性是出发菌株酶活性( 422u / ml )的341
10. The genes encoding pyruvate decarboxylase ( pdc ) and alcohol dehydrogenase ii ( adh ii ) were amplified from total dna of zymomonas mobilis by pcr . the genes of adhb andpdc were inserted into expression vector pse380 and then transformed into e . coli dh5a . the recombinant strains were induced to express adh ii and pdc with iptg
    本论文以zymomonasmobilisdna为模板， pcr扩增zymomonasmobilis中的乙醇脱氢酶基因( adhb )和丙酮酸脱羧酶基因( pdc ) ，分别构建表达质粒pse - adhb和pse - pdc并在大肠杆菌dh5中表达。
11. It was confirmed that the key enzyme of coq10 synthesis is p - hydroxybenzoate polyprenyltransferase , encoded by gene ubia in e . coli and lacks specificity to the aggregation length of its substrate ( polyisoprenyl pyrophosphate ) . if gene ubia can be introduced into psb and is high expression in psb , a recombinant strain producing coq10 could be obtained . consequently , the productive cost will decrease sharply
    业已证实coq _ ( 10 )生物合成的关键酶为对羟基苯甲酸聚异戊二烯焦磷酸转移酶(在大肠杆菌中该酶由ubia基因编码) ，该类酶对底物聚异戊二烯焦磷酸( ppp )的聚合长度并无特殊要求。
12. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium , then , induced by 0 . 5 % methanol . the expression protein was analyzed by sds - page after five days of induction . sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately , account for 30 % and 10 % of the total protein separately , which were purified using probond resin purification system , and obtained 15mg at levels above 0 . 75g / l and 7mg expression protein at levels above 0 . 35g / l separately once purification , the purity is both above 90 %
    筛选ade +表型转化子， 20mlbmmy摇瓶培养，用0 . 5甲醇诱导表达5天后， sds - page检测结果表明：选出的重组高效表达菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明显的表达特异条带，分子量分别为75kd和55kd ，分别占其总蛋白的30和10 ，经过probondresin镍亲和层析柱都得到了纯化，其纯度都在90以上，一次纯化分别可得到大约15mg和7mg表达蛋白，推知表达量分别高达0 . 75g l和0 . 35g l以上。
13. The recombinant plasmids pbmb121l , pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal , crylaclo and crylca from their original plasmid vectors to different plasmid vector . the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation . the transformation and expression properties of 8mb 171 were studied
    通过将苏云金芽胞杆菌杀虫晶体蛋白基因cry1aal 、 cry1ac10和cry1ca转移至不同质粒载体上，分别构建了重组质粒pbmb121l 、 pbmb9821l和pbmb986l ，并分别导入无质粒突变株bmb171 ，筛选得到携带相应icps基因的重组菌株。
14. However , the content of coq10 in wild strains of psb is limited after all . through construction of recombinant strain producing coq10 and optimization of the culture medium and fermentation conditions of a selected strain , the content of coq10 in psb will be increased markedly . it can not only remarkably strengthen the effect of psb feed additive , but also lay foundation for the exploitation of coq10
    但野生型psb菌株coq _ ( 10 )含量终究有限，如能选择合适菌株进行培养优化，并通过构建基因工程菌，使菌体中coq _ ( 10 )的含量成倍提高，则不仅可显著增强psb饲料添加剂的应用效果，更重要的是，为coq _ ( 10 )相关产品的研发奠定良好的基础。
15. This project is aimed at : ( 1 ) expressing melittin and megf in e . coli respectively ; ( 2 ) constructing a membrane - toxic immunotoxin by using melittin as toxic part and megf as target part ; ( 3 ) measuring the megf mrna expression abundance by quantitative competitive rt - pcr ; ( 4 ) checking the genetic stability of the recombinant strains constructed in the project ; ( 5 ) optimizing the fermentation conditions of the target strains by flask shake
    本课题以大肠杆菌( e . coli )宿主重组表达melittin和megf ；以melittin为毒性部分、 megf为靶向部分，构建膜毒性免疫毒素；用定量竞争rt - pcr法测定megf体内表达丰度；检验所构建的各重组菌株的遗传稳定性；采用摇瓶分析对各重组菌株的发酵条件进行了初步优化。
16. Pcr and enzyme acitivity check on chitin agar showed that the chitinase gene fragment existed and expressed in the wildtype strains . antagonistic activity test in vitro suggest that the transformants remained the ability to produce antibiotics . the recombinant strains showed an increased biocontrol efficacy against wheat take - all and rhizoctonia sheath blight in greenhouse
    对小麦全蚀病和纹枯病的盆栽试验结果表明工程菌株p25113一9的防病作用强于野生菌株，尤其是对小麦全蚀病的防治作用，其防治效果同野生菌株ap113和ap25相比，分别增长了22